KMID : 1038820240270010026
|
|
Pediatric Gastroenterology, Hepatology & Nutrition 2024 Volume.27 No. 1 p.26 ~ p.36
|
|
Mucosal Immunity Related to CD8+ T Lymphocytes in Children with Helicobacter pylori Gastritis
|
|
Yang Da-Hee
Lee Ha-Young Choi Woo-Hyuk Hyun Chang-Lim Kang Ki-Soo
|
|
Abstract
|
|
|
Purpose: We investigated the role of CD8+T cells as host immune factors in pediatric patients with Helicobacter pylori gastritis.
Methods: Gastric mucosal tissue and blood samples were collected from 39 children, including 11 children with H. pylori infection and 28 children as controls. Anti-CD8 and anti-T-bet antibodies were used for immunohistochemistry of the gastric mucosa. For the cell surface and intracellular staining, peripheral blood mononuclear cells were stained with anti-IL7R¥á, anti-CX3CR1, anti-CD8, anti-T-bet, and anti-IFN-¥ã antibodies. Cytokines of sera such as tumor necrosis factor alpha (TNF-¥á) and CX3CL1 were analyzed using enzyme- linked immunosorbent assay (ELISA).
Results: In the immunohistochemistry of gastric mucosa, the frequency of CD8+ and T-bet+ T cells cells was higher in the H. pylori-positive group than in the control group (26.9¡¾ 7.8% vs. 16.9¡¾3.3%, p<0.001; 5.0¡¾2.5% vs. 2.2¡¾0.7%, p=0.001). Between the control and H. pylori-positive groups, the frequency of IL-7R¥álowCX3CR1+ CD8+ and T-bet+ INF-¥ã+ CD8+ T cells were not significantly different between surface and intracellular staining, respectively (40.4¡¾24.0% vs. 38.2¡¾17.8%, p=0.914; 40.4¡¾24.0% vs. 38.2¡¾17.8%, p=0.914). In the ELISA, no significant differences in TNF-¥á and CX3CL1 concentrations were observed between the control and H. pylori-positive groups (34.3¡¾12.1 pg/mL vs. 47.0¡¾22.6 pg/mL, p=0.114/0.5¡¾ 0.1 pg/mL vs. 0.5¡¾0.1 pg/mL, p=0.188).
Conclusion: CD8+ T and Th1 cells, which secrete IFN-¥ã, might play important roles in the mucosal immunity of the stomach in children with H. pylori infection.
|
|
KEYWORD
|
|
Helicobacter pylori, Gastritis, Child, T-lymphocyte
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|